A multifunctional dihydromyricetin-loaded hydrogel for the sequential modulation of diabetic wound healing and glycemic control

作者：Hongyi Li, Huiyun Wen, He Zhang, Xiang Cao, Li Li, Xiaowen Hu, Yanmei Zhang, Xinkun Shen, Quazi T.H. Shubhra, Yang Hong, Xiaojun Cai · 发表于：Burns & Trauma · 年份：2025 · DOI：10.1093/burnst/tkaf024 · 被引用次数：53 · 研究领域：Medicinal plant effects and applications、Wound Healing and Treatments、Pomegranate: compositions and health benefits

Abstract Background The management of chronic diabetic wounds remains a formidable challenge in clinical practice. Persistent hyperglycemia triggers vasculopathy, neuropathy, and immune dysfunction, critically impeding wound repair. We developed a multifunctional hydrogel (DPFI) engineered for sequential therapeutic actions, including antibacterial, anti-inflammatory, antioxidant, pro-vascularization/epithelialization, and glycemic-regulating properties, to address these complications. Methods DPFI hydrogels were prepared by encapsulating dihydromyricetin (DMY) into aldehyde-functionalized Pluronic F127 micelles (DMY@PF127-CHO), followed by a Schiff base reaction with amine-rich polyethyleneimine (PEI), resulting in the formation of a hydrogel for controlled drug release. The antimicrobial, antioxidant, anti-inflammatory, pro-cellular proliferative, and angiogenic properties of the hydrogels were evaluated using various techniques, including structural characterization, bacterial live/dead staining, reactive oxygen species (ROS) assays, antioxidant enzyme assays, reverse transcription–polymerase chain reaction (RT–PCR), cellular immunofluorescence staining, scratch wound healing assays, and angiogenesis assays. In vivo, the effects of the hydrogel on wound healing and glycemic control were assessed in methicillin-resistant Staphylococcus aureus-infected mice with streptozotocin-induced diabetes. Results The hydrogel exhibits exceptional injectability, bioadhesion, and self-he...