Soil pH and total phosphorus regulate bacterial community assembly in slope restoration areas of the Tibetan Plateau's metal mining areas

作者：Huanyu Zhou, Xiaotong Liu, Xianlei Gao, Yan Wang, Liming Ye, Jun Wu, Mingxue Xiang · 发表于：Environmental Research · 年份：2025 · DOI：10.1016/j.envres.2025.121432 · 被引用次数：7 · 研究领域：Microbial Community Ecology and Physiology、Mining and Resource Management、Metal Extraction and Bioleaching

Microbial community development is a crucial aspect of soil restoration. The employment of frame beams in conjunction with external soil has demonstrated efficacy in the rehabilitation of degraded roadside ecosystems within mining regions. Nonetheless, the effects of frame beams on the composition and stability of soil bacterial communities remain inadequately comprehended. We conducted a one-time soil sampling on a three-year restored slope in a large-scale metal mining area on the Tibetan Plateau, providing a snapshot of the current conditions and evaluating the restoration progress. Frame beams with external soil covers were applied at three different altitudes: A1 (4800-5000 m), A2 (4500-4700 m), and A3 (4200-4400 m). Restoration significantly altered bacterial community composition compared with controls. Proteobacteria had a higher relative abundance in the restoration area (average: 31.16 %), whereas Acidobacteriota were more abundant in the control area (average: 24.68 %). In the restoration area, soil bacterial α-diversity increased as elevation decreased, with the Shannon index rising from 5.34 (A1) to 5.82 (A3), suggesting that bacterial communities at higher altitudes are more sensitive to environmental conditions. Species turnover was the primary driving factor of β-diversity, accounting for 96.26 % under A1, 94.71 % under A2, and 91.94 % under A3, respectively. The nearest taxon index of bacterial communities shifted from negative to positive along the elevation...