Biological delignification and anaerobic fermentation of wheat straw: A promising approach for sustainable utilization of crop straw bioresources

作者：Mudasir Nazar, Jipeng Tian, Xin Wang, Siran Wang, Nazir Ahmad Khan, Yunhui Cheng, Wenjie Zhang, Nengxiang Xu, Beiyi Liu, Chenglong Ding · 发表于：Industrial Crops and Products · 年份：2025 · DOI：10.1016/j.indcrop.2025.120839 · 被引用次数：23 · 研究领域：Biofuel production and bioconversion、Bioenergy crop production and management、Ruminant Nutrition and Digestive Physiology

Agricultural crop residues, such as wheat straw, represent a renewable and valuable bioresource. However, their utilization is often constrained by the recalcitrant structure, primarily due to severe lignification, leading to resource wastage and environmental pollution. This study offers a promising approach for the hygienic preservation and efficient utilization of wheat straw through laccase mediated delignification. The effects of laccase pretreatment on anaerobic preservation, structural composition, biodegradation, and in vitro digestibility were investigated. Wheat straw was ensiled for 120 days after four pretreatments: untreated (control), or pretreated with Lactobacillus plantarum and hemicellulase (LPH), hemicellulase and laccase (HL), and a combination of L. plantarum , hemicellulase and laccase (LPHL). Laccase pretreatment significantly degraded lignin, enhancing cellulose and hemicellulose hydrolysis. The LPHL pretreatment significantly reduced silage pH (<4.10) and lignin content, and preserved higher (p < 0.05) concentrations of water-soluble carbohydrates (15.06 g/kg dry matter). Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction confirmed substantial physico-chemical alterations in silage composition, particularly in HL and LPHL treated groups. Notably, HL treatment improved in vitro neutral detergent fiber (36.56 %) and acid detergent fiber (37.91 %, p < 0.05) degradation. Moreover, delignified silage (HL and LPHL) ...