Integrative analysis of transcriptome and metabolome reveal molecular mechanism of tolerance to salt stress in rice

作者：Rui Deng, Yao Li, Naijie Feng, Dianfeng Zheng, Aaqil Khan, Youwei Du, Jianqin Zhang, Zhiyuan Sun, Jia-Shuang Wu, Yingbin Xue, Zihui Huang · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-06300-8 · 被引用次数：15 · 研究领域：Plant Stress Responses and Tolerance、Plant responses to water stress、Photosynthetic Processes and Mechanisms

BACKGROUND: Salt stress is considered to be one of the major abiotic stresses influencing rice growth and productivity. To improve rice crop productivity in saline soils, it is essential to choose a suitable variety for mitigating salt stress and gain a deep understanding of the underlying mechanisms. The current study explored the salt tolerance mechanism of wild rice 'HD96-1 (salt resistive)' and conventional rice 'IR29 (salt sensitive)' by evaluating morph-physiological, transcriptomic, and metabolomic approaches. RESULTS: , less H₂O₂, and lower electrolyte leakage under salt stress compared with IR29. Transcriptomic and metabolomic data showed that the expression of NHXs in IR29 was significantly down-regulated under salt stress, leading to a large accumulation of Na⁺ in the cytoplasm, and that the expression of CHLH, PORA, and PORB was significantly down-regulated, inhibiting chlorophyll synthesis. HD96-1 maintained the balance of Na⁺ and K⁺ by increasing the expression of NHX4, and there was no significant change in the expression of genes related to chlorophyll synthesis, which made HD96-1 more resistant to salt stress than IR29. In addition, HD96-1 inhibited the excessive synthesis of hydrogen peroxide (H₂O₂) and alleviated oxidative damage by significantly down-regulating the expression of ACX4 under salt stress. HD96-1 promoted the accumulation of isoleucine by up-regulating genes of branched-chain amino acid aminotransferase 2 and branched-chain amino acid aminotra...