Microfluidic magnetic droplet-based chemiluminescence enzyme immunoassay for multiplex sepsis biomarker screening

作者：Jiayue Huang, Jinbo Li, Nan Wu, Xin Yang, Lexiang Zhang, Feika Bian, Dan Hu · 发表于：The Analyst · 年份：2025 · DOI：10.1039/d4an01253d · 被引用次数：5 · 研究领域：Biosensors and Analytical Detection、Innovative Microfluidic and Catalytic Techniques Innovation、Cystic Fibrosis Research Advances

Poisson distribution in the generated droplets provide a reliable quantitative strategy for detecting rare targets. This method achieves high sensitivity, low interference, and simultaneous detection with satisfactory specificity of various sepsis biomarkers, such as procalcitonin (PCT), interleukin-6 (IL-6), and C-reactive protein (CRP). These features demonstrate that the microfluidic droplet-based CLIA method has great potential for broader applications in multiplex biomolecule detection and early disease diagnosis.