Interactions between starvation, cold stress and water velocities on immunological and metabolic responses of large yellow croaker

作者：Chengzhang Huang, Huirong Lv, Xu‐Ming Huang, Xiaolong Yin, Zhangjie Chu, Jungyeol Park, Jun‐Wook Hur, Yang Gao, Weiye Li · 发表于：Aquaculture Reports · 年份：2025 · DOI：10.1016/j.aqrep.2025.102746 · 被引用次数：11 · 研究领域：Aquaculture Nutrition and Growth、Aquaculture disease management and microbiota、Physiological and biochemical adaptations

Research shows that natural starvation during cold months may enhance the croaker's cold tolerance, yet the high mortality rates persist, indicating that factors like water velocity may also play a critical role. This study investigates how low temperature, starvation and water velocities affect the croaker's survival, focusing on antioxidant capacity, metabolic, transcriptomic and microbiota changes, aiming to improve management strategies for this economically important species in aquaculture. 400 large yellow croakers were divided into fed and starved groups to assess cold tolerance under gradually decreasing temperatures, and swimming endurance under increased water velocities up to 0.35 m/s. The fish were subsequently subjected to simulated overwintering in controlled flow conditions at different velocities to observe survival rates. Samples from the fish, including liver and intestines, were collected for antioxidant capacity, transcriptomic, metabolomic and microbiota analyses. Our results demonstrate that starvation enhances cold tolerance in large yellow croakers, but reduces swimming endurance significantly, suggesting a complex interplay between metabolic preservation and physical capability. Transcriptomic analyses revealed a significant shift towards fatty acid oxidation and down-regulation of steroid biosynthesis pathways, particularly under combined stress conditions. Moreover, the excessive up-regulation of lipid metabolism led to the accelerated production of...