The immunomodulation of outer membrane vesicles from Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in Pacific white shrimp Litopenaeus vannamei

作者：Lihan Wang, Jinyu Yang, Shengwen Calvin Li, Zhe Qu, Mengqiang Wang · 发表于：Fish & Shellfish Immunology · 年份：2025 · DOI：10.1016/j.fsi.2025.110274 · 被引用次数：8 · 研究领域：Aquaculture disease management and microbiota、Invertebrate Immune Response Mechanisms、Aquaculture Nutrition and Growth

Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease ( Vp AHPND) is a significant bacterial pathogen to Litopenaeus vannamei aquaculture with a substantial economic burden. Outer membrane vesicles (OMVs) released by Gram-negative pathogenic bacteria play complex roles in the modulation on host's immune response. To elucidate the potential roles of Vp AHPND-OMVs on L. vannamei innate immune responses, this study investigated the immune responses and molecular mechanisms induced by Vp AHPND-OMVs in hepatopancreas using transcriptomic and proteomic analysis. Shrimps were fed diets supplemented with 30 μg kg −1 (T1 group) or 60 μg kg −1 Vp AHPND-OMVs (T2 group), and the control group was fed a normal diet (CK group). Neither growth rate and hepatopancreas histological structure were affected by Vp AHPND-OMVs. The most pronounced changes in the activities of immune-related enzymes, including lysozyme, superoxide dismutase, alkaline phosphatase and glutathione S-transferase, were observed at 7 and 14 days of the experiment, which suggested that Vp AHPND-OMVs can rapidly and significantly enhance the activity of immune enzymes within a short period. The transcription levels of genes associated with immune and pathogen defense were significantly downregulated in the T1 and T2 groups including heat shock 70 kDa protein cognate 4-like (HSP70), beta-1,3-glucan-binding protein-like (GNBP1), C-type mannose receptor 2-like (MRC2), penaeidin-3a-like (PEN-3), and chitinase...