Escin Rescues Blood-brain Barrier and Inhibits NLRP3 Inflammasome-mediated Pyroptosis in Rats with Superior Sagital Sinus Thrombosis

作者：Min Li, Xinjian Liu, Xinyu Zhou, Ran Meng, Xunming Ji · 发表于：International Journal of Medical Sciences · 年份：2025 · DOI：10.7150/ijms.102624 · 被引用次数：6 · 研究领域：Cerebral Venous Sinus Thrombosis、Inflammasome and immune disorders、Ocular Diseases and Behçet’s Syndrome

Objective: The purpose of this study is to investigate the therapeutic value of escin and its potential mechanisms in rats with superior sagittal sinus thrombosis (SSST).Methods: The motor function of rats was assessed using the open field, balance beam, and rotarod tests following one week of escin treatment.Laser Speckle Contrast Imaging (LSCI) was used to evaluate the blood flow in the superior sagittal sinus.Evans blue staining was used to assess the permeability of blood-brain barrier.The protein expression levels of Occludin, ZO-1 and MMP-9 was analyzed by Western blotting to assess blood-brain barrier disruption.Iba1, CD68 and NLRP3 immunofluorescent staining was conducted to evaluate the activation of microglia and NLRP3 inflammasome.The protein expression levels of NLRP3, Caspase-1, IL-1β, IL-18 and GSDMD was analyzed by Western blotting to assess NLRP3 inflammasome activation and pyroptosis.Immunohistochemistry for NeuN was used to evaluate the neuronal pyroptosis.Results: Rats with SSST exhibited decreased movement distance, duration and latency to fall, as well as increased balance latency compared to sham rats, indicating its motor dysfunction.Escin treatment alleviated the motor dysfunction in rats with SSST.LSCI revealed a decrease in the blood flow in the superior sagittal sinus of rats with SSST.There was no discernible change in blood flow between SSST rats treated with escin and those not, suggesting that escin did not promote recanalization of the superior...