Esculin promotes skin wound healing in mice and regulates the Wnt/β-catenin signaling pathway

作者：Mian Xu, Mengsi Zhang, Jingjing Wu, Jinmeng Wang, Haobo Wu, Xiaoru Xu · 发表于：CytoJournal · 年份：2025 · DOI：10.25259/cytojournal_184_2024 · 被引用次数：5 · 研究领域：Wound Healing and Treatments、Wnt/β-catenin signaling in development and cancer、Pressure Ulcer Prevention and Management

Objective Previous studies reported that esculin could protect against renal ischemia-reperfusion injury and liver injury, but its mechanism of action in skin wound healing is unclear. The Wnt/β-catenin signaling pathway plays a positive role in the wound healing process. This study aimed to investigate the effects of esculin on the rate and quality of skin wound healing in mice and explore its regulatory role in the Wnt/b-catenin signaling pathway. Material and Methods Circular full-thickness skin wounds with a diameter of 8 mm were created on the backs of C57BL/6 mice, which were administered with 20 and 40 mg•kg −1 esculin through gastric lavage. Wound healing was monitored, and samples collected on day 14 were analyzed through hematoxylin-eosin and Masson staining to assess granulation tissue formation and collagen deposition. Immunohistochemistry, immunofluorescence, and Western blot evaluated markers of collagen synthesis, proliferation, angiogenesis, and proteins in the Wnt/b-catenin signaling pathway. National institutes of health/3T3 cells treated with esculin (50 and 200 μM) were analyzed for proliferating cell nuclear antigen (PCNA) expression to assess proliferative activity. Results Compared with the model group, the esculin-treated groups exhibited significantly enhanced wound healing ( P < 0.05), increased skin epithelial thickness ( P < 0.01), and promoted extracellular matrix formation in mice. In addition, esculin significantly raised type I collagen a...