Aptamer-based biosensors for wastewater surveillance of influenza virus, SARS-CoV-2, and norovirus: A comprehensive review

作者：Yilei Wang, Mohan Amarasiri, Wakana Oishi, Masayasu Kuwahara, Yuka Kataoka, Hiroki Kurita, Fumio Narita, Rong Chen, Qian Li, Daisuke Sano · 发表于：Water Research · 年份：2025 · DOI：10.1016/j.watres.2025.123484 · 被引用次数：11 · 研究领域：SARS-CoV-2 detection and testing、Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection

• Biosensors hold promise for public health monitoring through WBE. • Aptamers are highly effective elements for the detection of pathogenic biomarkers. • Aptamer biosensors achieve femtomolar detection limits in wastewater. • This review identifies current challenges in translating lab findings to practice. • Recommendations provided for future research in aptamer-based WBE applications. Wastewater-based epidemiological (WBE) surveillance has emerged as a crucial tool for monitoring infectious diseases within communities. However, its broader application is frequently constrained by the high costs, labor-intensive processes, and extended timeframes required for sample collection, transportation, and processing. Aptamer-based biosensors offer a promising alternative, leveraging the specific binding properties of aptamers to biomolecules for the on-site and rapid quantification of disease biomarkers in wastewater. This review systematically evaluates recent advancements in the application of aptamer-based biosensors for the detection of key pathogens, including SARS-CoV-2, influenza viruses, and norovirus, within wastewater matrices. The discussion encompasses the technical stability and reliability of signal transmission associated with these biosensors, as well as the current challenges faced in real-world implementation. Noteworthy progress has been made in the development of these biosensors for WBE, achieving detection limits as low as femtomolar (fM) levels in buffer and...