Diverse Acinetobacter species and Plasmid-Driven spread of carbapenem resistance in pharmaceutical settings in China

作者：Jintao He, Liang Hong, Meijun Song, Yisha Zhang, Wang Zhang, Linghong Zhang, Danyan Zhou, Zixin Chen, Yunsong Yu, Huan Chen, Xiaoting Hua · 发表于：Environment International · 年份：2025 · DOI：10.1016/j.envint.2025.109373 · 被引用次数：6 · 研究领域：Antibiotic Resistance in Bacteria、Vibrio bacteria research studies、Pharmaceutical and Antibiotic Environmental Impacts

• A high diversity of Acinetobacter species was found in pharmaceutical settings. • No Acinetobacter transmission events occurred between pharmaceutical environments and clinics. • The NDM-1 plasmids resemble those from clinic and confer resistance to carbapenems. • Two novel Acinetobacter species were identified and characterized. • A. yuyunsongii sp. nov. strain harbored bla OXA-58 and exhibited MDR phenotype. Acinetobacter species have emerged as a significant public health concern due to their remarkable capacity to acquire antimicrobial resistance. Environmental reservoirs play a crucial role in spreading antimicrobial resistance genes and potentially pathogenic bacteria to clinical settings. However, most studies on nonhuman isolates have focused on a limited sample size. Comprehensive population sampling on One Health principles is essential to monitor the antibiotic resistome and virulome in Acinetobacter spp. Here, we identified three carbapenems-resistant Acinetobacter spp. isolates harboring bla NDM-1 and discovered two novel Acinetobacter species in pharmaceutical production environments. A total of 94 Acinetobacter spp. strains were isolated from pharmaceutical production environments across 17 cities in China, forming 17 distinct Acinetobacter clusters comprising two novel species and 15 previously known species. Phylogenetic analysis indicated that Acinetobacter spp. isolated from pharmaceutical environments are predominantly confined to these settings. Genomic...