CPCGI Alleviates Neural Damage by Modulating Microglial Pyroptosis After Traumatic Brain Injury

作者：Lu‐Lu Yu, Lei Sun, Tingting Yu, Anchen Guo, Jian‐Ping Wu, Junmin Chen, Qun Wang · 发表于：CNS Neuroscience & Therapeutics · 年份：2025 · DOI：10.1111/cns.70322 · 被引用次数：7 · 研究领域：Inflammasome and immune disorders、Spinal Cord Injury Research、Neuroinflammation and Neurodegeneration Mechanisms

BACKGROUND: Traumatic brain injury (TBI) is a major global cause of mortality and long-term disability, with limited therapeutic options. Microglial pyroptosis, a form of programmed cell death associated with inflammation, has been implicated in exacerbating neuroinflammation and secondary injury following TBI. Compound porcine cerebroside ganglioside injection (CPCGI) has shown anti-inflammatory and antioxidant properties, but its effects on pyroptosis remain unexplored. This study investigates the role of CPCGI in TBI and its underlying mechanisms. METHODS: A controlled cortical impact (CCI) model was utilized to establish TBI in vivo, while lipopolysaccharide (LPS) was used in vitro to induce microglial activation that mimicked TBI conditions. The effects of CPCGI on microglial pyroptosis and inflammatory cytokines were analyzed through immunofluorescence, flow cytometry, western blotting, and quantitative real-time PCR (qRT-PCR). The involvement of the NLRP3 inflammasome in CPCGI's mechanism was examined using NLRP3 overexpression or the NLRP3 agonist BMS-986299. A microglia-neuron interaction model was created, and neuronal injury was assessed with the Cell Counting Kit-8 and Fluoro-Jade C (FJC). RESULTS: Treatment with CPCGI resulted in significant improvement in the neurobehavioral outcomes, reduced lesion volume, and decreased neuronal loss following TBI. Notably, TBI induced microglial pyroptosis and the release of pro-inflammatory cytokines, while CPCGI inhibited mi...