Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells

作者：Nemanja Vukašinović, Che‐Wei Hsu, M. Marconi, Shaopeng Li, Christopher B. Zachary, Rachel Shahan, Pablo Szekley, Ziv Aardening, Isabelle Vanhoutte, Qian Ma, Lucrezia Pinto, Pavel Krupař, Н В Герман, Jingyuan Zhang, Claire Simon--Vezo, Jessica Pérez‐Sancho, Pepe Cana Quijada, Qianzi Zhou, Laura R. Lee, Jianghua Cai, Emmanuelle Bayer, Matyáš Fendrych, Elisabeth Truernit, Yu Zhou, Sigal Savaldi‐Goldstein, Krzysztof Wabnik, Trevor M. Nolan, Eugenia Russinova · 发表于：Cell · 年份：2025 · DOI：10.1016/j.cell.2025.02.011 · 被引用次数：31 · 研究领域：Plant Molecular Biology Research、Plant nutrient uptake and metabolism、Chromosomal and Genetic Variations

Brassinosteroid hormones are positive regulators of plant organ growth, yet their function in proliferating tissues remains unclear. Here, through integrating single-cell RNA sequencing with long-term live-cell imaging of the Arabidopsis root, we reveal that brassinosteroid activity fluctuates throughout the cell cycle, decreasing during mitotic divisions and increasing during the G1 phase. The post-mitotic recovery of brassinosteroid activity is driven by the intrinsic polarity of the mother cell, resulting in one daughter cell with enhanced brassinosteroid signaling, while the other supports brassinosteroid biosynthesis. The coexistence of these distinct daughter cell states during the G1 phase circumvents a negative feedback loop to facilitate brassinosteroid production while signaling increases. Our findings uncover polarity-guided, uneven mitotic divisions in the meristem, which control brassinosteroid hormone activity to ensure optimal root growth.