Increased maternal consumption of methionine as its hydroxyl analog improves placental angiogenesis and antioxidative capacity in sows

作者：Rui Zhou, Shanshan Lai, Peiqiang Yuan, Zhe Li, Lunxiang Yang, Yves Mercier, Liang‐Hao Hu, Xiaoling Zhang, Lun Hua, Yong Zhuo, Shengyu Xu, Lin Yan, Bin Feng, Lianqiang Che, De Wu, Zhengfeng Fang · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2025 · DOI：10.1186/s40104-025-01159-z · 被引用次数：6 · 研究领域：Folate and B Vitamins Research、Sulfur Compounds in Biology、Pregnancy and preeclampsia studies

BACKGROUND: Previous evidence suggests that methionine (Met) consumption can promote placental angiogenesis and improve fetal survival. To investigate the mechanisms by which increased levels of Met as hydroxyl-Met (OHMet) improve placental function, forty sows were divided into four groups and fed either a control diet, or diets supplemented with 0.15% OHMet, 0.3% OHMet or 0.3% Met (n = 10). Placentas were collected immediately after expulsion, and extracted proteins were analyzed by tandem mass tag based quantitative proteomic analysis. RESULTS: We found that 0.15% OHMet consumption significantly increased placental vascular density compared with the control. Proteomic analysis identified 5,136 proteins, 87 of these were differentially expressed (P < 0.05, |fold change| > 1.2). Enriched pathways in the Kyoto Encyclopedia of Genes and Genomes for 0.15% OHMet vs. control and 0.15% OHMet vs. 0.3% OHMet were glutathione metabolism; for 0.15% OHMet vs. 0.3% Met, they were NOD-like receptor signaling and apoptosis. Further analysis revealed that 0.15% OHMet supplementation upregulated the protein expression of glutathione-S-transferase (GSTT1) in placentas and trophoblast cells compared with the control and 0.3% OHMet groups, upregulated thioredoxin (TXN) in placentas and trophoblast cells compared with the 0.3% OHMet and 0.3% Met groups, and decreased reactive oxygen species (ROS) levels in trophoblast cells compared with other groups. In contrast, sows fed 0.3% OHMet or 0.3% Me...