Comparative genomics and phylogenetic analysis of mitochondrial genomes of Neocinnamomum

作者：Wenjun Zhu, Di Zhang, Wenbin Xu, Yi Gan, Jiepeng Huang, Yanyu Liu, Yun‐Hong Tan, Yu Song, Peiyao Xin · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-06238-x · 被引用次数：11 · 研究领域：Genomics and Phylogenetic Studies、Photosynthetic Processes and Mechanisms、Plant Diversity and Evolution

BACKGROUND: Neocinnamomum plants are considered a promising feedstock for biodiesel in China, due to the richness in long-chain fatty acids (LCFAs) found in their seeds. However, the mitochondrial genome (mitogenome) of this genus has not yet been systematically described, and the exploration of species relationships within this genus using mitogenome sequences is also an uncharted territory. This has hindered our understanding of mitogenome diversity and the evolutionary relationships within Neocinnamomum. RESULTS: In this study, a total of 24 individuals representing seven distinct taxa from the genus Neocinnamomum were subjected to Illumina sequencing, and the species N. delavayi was sequenced using Oxford Nanopore sequencing technology. We successfully assembled the mitogenome of N. delavayi, which is 778,066 bp in size and exhibits a single circular structure. The analysis identified 659 dispersed repeats, 211 simple sequence repeats (SSRs), and 30 tandem repeats within the mitogenome. Additionally, 37 homologous fragments, totaling 9929 bp, were found between the mitogenome and the plastid genome (plastome). The codons of 41 protein-coding genes (PCGs) had a preference for ending in A/T, and the codon usage bias of the majority of these genes was influenced by natural selection pressures. Comparative genomic analysis revealed low collinearity and significant gene rearrangements between species. Phylogenetic analysis resulted in the classification of Neocinnamomum into s...