Diversity of intestinal microbiota and inflammatory cytokines after severe trauma

作者：Changqing Liu, Jie Yang, Hongfei Ren, Guang-neng Liao, Zhe Yin, Shi-lin Gao, Qiujing Du, Xing-zhu Yuan, Hanif Ullah, Ka Li · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-92212-4 · 被引用次数：5 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Enhanced Recovery After Surgery

Accumulating evidence has reported that the intestinal microbiota could play important roles in the occurrence and progression of severe trauma. However, the hypothesized potential targeted intestinal microbiota to mediate and regulate the levels of inflammatory cytokines and promote rapid recovery of body after severe trauma remains unclear. This study was aimed to explore the changes and correlation of intestinal microbiota and inflammatory cytokines in rats with severe crush and fracture trauma. The controlled laboratory study design was used, and a crush and fracture severe trauma rat model was established. 16S rRNA high-throughput gene sequencing and ELISA were used to analyze the changes in intestinal microbiota and inflammatory cytokines within one week after trauma. The correlation between intestinal microbiota and inflammatory cytokines was also analyzed. Loss of overall diversity and expansion of intestinal microbiota in the rats due to severe trauma was observed. Specifically, there was a significant increase in the abundance of Muribaculaceae [LDA (Linear Discriminant Analysis)-value = 4.814, P = 0.014] after severe trauma, while Prevotella (LDA-value = 5.235, P = 0.020) and Alloprevotella (LDA-value = 4.443, P = 0.015) were slightly lower in the trauma group than in the control group. The levels of inflammatory cytokines (IL-1α, IL-6, IL-8 and TNF-α) in the trauma group decreased from the first day to the third day and continued to increase until one week after t...