Ubiquitin Ligase Gene OsPUB57 Negatively Regulates Rice Blast Resistance

作者：Jian Zhang, Qiang Du, Yu Wu, Mengyu Shen, Furong Gao, Zhilong Wang, Xiuwen Xiao, Wenbang Tang, Qiuhong Chen · 发表于：Plants · 年份：2025 · DOI：10.3390/plants14050758 · 被引用次数：2 · 研究领域：Ubiquitin and proteasome pathways、Photosynthetic Processes and Mechanisms、Plant Gene Expression Analysis

The ubiquitination and degradation of proteins are widely involved in plant biotic and abiotic stress responses. E3 ubiquitin ligases play an important role in the ubiquitination of specific proteins. In this study, we identified the function of a U-box E3 ubiquitin ligase gene OsPUB57 in rice. Expression analyses revealed that OsPUB57 was mainly expressed in the aboveground part of rice. Drought, salt, cold, JA (jasmonic acid), PAMPs (pathogen-associated molecular patterns) or Magnaportheoryzae treatment could significantly suppress the expression of OsPUB57 in rice. Compared with wild-type plants, OsPUB57-overexpressing plants showed a decrease in resistance to M. oryzae, while the mutant plants exhibited an enhancement of M. oryzae resistance. The expression level detection indicated that OsPUB57 negatively regulates rice blast resistance, probably by down-regulating the expression of the defense-related genes OsPR1a and OsAOS2. This study provides a candidate gene for the genetic improvement of rice blast resistance.