Enhancing naphthenic acid attenuation in mesocosm wetlands: The role of temperature, plant species, and microbial communities

作者：Kaitlyn E. Trepanier, Amy‐lynne Balaberda, Ian J. Vander Meulen, Jason M. E. Ahad, Sara Correa‐García, Simon Morvan, Marie‐Josée Bergeron, Dilini Atugala, Lisa M. Gieg, John V. Headley, Étienne Yergeau, Christine Martineau, Dani Degenhardt · 发表于：Water Environment Research · 年份：2025 · DOI：10.1002/wer.70048 · 被引用次数：7 · 研究领域：Petroleum Processing and Analysis、Hydrocarbon exploration and reservoir analysis、Microbial bioremediation and biosurfactants

Abstract Oil sands process‐affected water (OSPW) is a by‐product of bitumen extraction from oil sands surface mining in Alberta, Canada. A major group of organics in OSPW known as naphthenic acid fraction compounds (NAFCs) are of concern due to their persistence and toxicity. Constructed wetland treatment systems have emerged as potential biological treatment approaches for reducing NAFC concentrations within OSPW. In this study, greenhouse‐scale mesocosms simulating a constructed wetland consisting of coarse sand tailings (CST) and OSPW were used to evaluate the ability of Scirpus microcarpus , Triglochin maritima , and unplanted controls to attenuate NAFCs under spring/fall and summer temperatures (10°C/5°C and 20°C/10°C day/night). Overall, in this mesocosm system, NAFC attenuation was similar regardless of different design parameters such as plant type, plant presence, and temperature. By the end of the study, NAFCs attenuation was 30% to 50% lower than the initial OSPW depending on plant species, plant presence, and temperature. The relative abundance of the acutely toxic O 2 ‐NAFCs decreased over time, with an increase in the less toxic O 3 , O 4 , and SO 3 classes. Various hydrocarbon‐degrading microbial families such as Comamonadaceae and Xanthobacteraceae were found to be dominant in OSPW, while cyanobacteria ( Trichormus ) were enriched in the CST. Principal component analysis indicated that only time led to distinct clusters for NAFC composition, while plant type, ...