Effects of dietary supplementation of creatine on fetal development in gilts at d 60 and d 90 of gestation

作者：Arianna N. Lopez, M. Olivarez, Claire Stenhouse, Robyn M Moses, Makenzie G Newton, Nirvay Sah, Heewon Seo, Joseph C. Cain, Carli Lefevre, A. Catharine Ross, Patrick Ryan, Jeffrey Wiegert, Guoyao Wu, Gregory A. Johnson, Fuller W. Bazer · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2025 · DOI：10.1186/s40104-025-01166-0 · 被引用次数：4 · 研究领域：Reproductive Physiology in Livestock、Animal Behavior and Welfare Studies、Veterinary Medicine and Surgery

BACKGROUND: The creatine-creatine kinase-phosphocreatine (Cr-CK-PCr) system maintains intracellular ratios of ATP/ADP for support of cellular functions and has been characterized at the placental-uterine interface of rodents, primates, swine and sheep, and thus may support fetal development. This study determined effects of dietary supplementation of creatine (Cr) to gestating gilts on fetal development, the number and ratio of primary and secondary muscle fibers, and on protein expression in endometrium and fetal biceps-femoris muscle, respectively in fetal pigs on d 60 and d 90 of gestation. METHODS: Reproductively mature gilts were synchronized to estrus using Matrix, observed for estrus (d 0), and artificially inseminated 12 h and 36 h later. Gilts were individually housed and fed 0.86 kg of 14% crude protein diet twice daily that meets nutritional requirements for pregnant gilts. Gilts were assigned to either basal diet control (CON) group, or Cr supplemented group (provided 30 g Cr monohydrate daily) from d 10 to either d 60 or d 90 of gestation. Gilts were euthanized and hysterectomized on either d 60 or d 90 of gestation. These protocols were completed in two replicates, as gilts were bred in spring and euthanized in summer or bred in fall and euthanized in winter (n = 20 gilts/replicate). Litter size, crown-rump length, sex, and fetal weight was recorded. Three female and male fetuses closest to mean litter weight were selected to assess effects of treatment on weigh...