Effects of Short-Term Traffic-Related Air Pollution Exposure on Nasal Microbiome in Young Healthy Adults: A Randomized Crossover Controlled Trial

作者：Luwei Qin, Jingqi Pan, Demin Feng, Bingqing Yu, Shunyu Li, Xingyu Liu, Yuefei Jin, Shenshen Zhu, Weidong Wu, Wenjie Yang · 发表于：Toxics · 年份：2025 · DOI：10.3390/toxics13030180 · 被引用次数：3 · 研究领域：Indoor Air Quality and Microbial Exposure、Pediatric health and respiratory diseases、Sinusitis and nasal conditions

Traffic-related air pollution (TRAP) remains a concern for public health. However, the exact mechanisms through which TRAP affects the respiratory system are still not fully understood. This study aimed to investigate the nasal microbiome change in healthy adults after short-term exposure to TRAP, contributing to the understanding of the adverse health effects associated with TRAP. A randomized crossover controlled trial was conducted from 9 March to 30 March 2024 among college students aged 19–24 years. Twenty healthy students were recruited through a baseline questionnaire survey and randomly assigned into two groups. One group followed a crowed-testing procedure: the park portion, a three-week washout period, and then the road portion, while the other group experienced the opposite procedure. Both groups were fully exposed to either a park environment or a road environment with high traffic volume. Nasal mucus samples were collected from the participants at the end of the trial, and then 16SrRNA sequencing was performed to analyze the differences in compositional structure and diversity of the nasal microbiome when volunteers were exposed to different levels of TRAP. The α-diversity indices, including the Chao1 index (p = 0.0097), observed species index (p = 0.0089), and Faith’s PD index (p = 0.0255), demonstrated a significant increase in the nasal microbiome of healthy adults following short-term exposure to TRAP. Visualization through a two-dimensional NMDS plot (stress...