circZNF707 promoted glycolysis and tumor progression through miR-668-3p-PFKM axis in NSCLC

作者：Wei Chen, Shuai Fang, Xianqiao Wu, Tianzheng Fang, Ziyuan Chen, Wang Su, Yuchao Zhu, Xiaodong Zhao, Chengwei Zhou · 发表于：European journal of medical research · 年份：2025 · DOI：10.1186/s40001-025-02359-z · 被引用次数：5 · 研究领域：Circular RNAs in diseases、MicroRNA in disease regulation、Connective Tissue Growth Factor Research

BACKGROUND: Circular RNA (circRNA) plays an important regulatory role in the development of human malignancies, but the potential mechanisms of circRNA in non-small cell lung cancer (NSCLC) remain largely unknown. METHODS: Microarray analysis was used to test for circRNAs differing in expression between NSCLC tumors and healthy adjacent tissues. Using qRT-PCR, the expression of circZNF707 was determined. Through a number of loss-of-function and gain-of-function investigations, the biological behavior of NSCLC cells was evaluated. Finally, tests using Western blotting, RIP, qRT-PCR, and luciferase reporter gene detection and rescue assays revealed the potential mechanism of circZNF707. RESULTS: Increased expression of circZNF707 was found in NSCLC tissues. Functionally, circZNF707 enhances proliferation, migration, invasion, and glycolysis of NSCLC cells. Mechanistically, circZNF707 can upregulate PFKM by acting as a sponge for miR-668-3p, thus contributing to the progression of NSCLC. CONCLUSIONS: Through the circZNF707/miR-668-3p/PFKM axis, upregulation of circZNF707 promotes tumor development. CircZNF707 may provide new insights into the treatment and diagnosis of NSCLC.