Analysis of single-cell and spatial transcriptomics in TNBC cell-cell interactions

作者：Yan Xin, Qiji Ma, Qiang Deng, Tielin Wang, Dongxu Wang, Gang Wang · 发表于：Frontiers in Immunology · 年份：2025 · DOI：10.3389/fimmu.2025.1521388 · 被引用次数：14 · 研究领域：Single-cell and spatial transcriptomics、Immune cells in cancer、Cancer Genomics and Diagnostics

Triple-negative breast cancer (TNBC) is a highly malignant tumor in women, characterized by high morbidity, mortality, and recurrence rates. Although surgical treatment, radiotherapy, and chemotherapy are the mainstays of current treatment methods, the high heterogeneity of TNBC results in unsatisfactory outcomes with low 5-year survival rates. Rapid advancements in omics technology have propelled the understanding of TNBC molecular biology. The emergence of single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) has significantly enhanced knowledge of tumor heterogeneity and the distribution, functionality, and intercellular interactions of various cell types within the tumor microenvironment, including tumor cells, T cells, B cells, macrophages, and fibroblasts. The present study provides an overview of the technical characteristics of scRNA-seq and ST, highlighting their applications in exploring TNBC heterogeneity, cell spatial distribution patterns, and intercellular interactions. This review aims to enhance the comprehension of TNBC at the cellular level for the development of effective therapeutic targets.