Comparative evaluation of QuantiFERON-TB gold in-tube plus for Mycobacterium tuberculosis infection among adolescents in China

作者：Peng Lü, L. Sánchez Martínez, Hao Xue, Qiao Liu, Xiaoyan Ding, Jingjing Pan, Hui Ding, Wei Lü, Limei Zhu, Jianming Wang · 发表于：BMC Public Health · 年份：2025 · DOI：10.1186/s12889-025-21954-7 · 被引用次数：3 · 研究领域：Tuberculosis Research and Epidemiology、Infectious Diseases and Tuberculosis、Mycobacterium research and diagnosis

BACKGROUND: No head-to-head studies have simultaneously compared the performances of QuantiFERON-TB Gold In-Tube (QFT-GIT), QuantiFERON-TB Gold Plus (QFT-Plus), ESAT6-CFP10 (EC) skin test, and Tuberculin skin test (TST) in adolescents. This study aimed to conduct a comparative assessment of QFT-GIT and QFT-Plus for detecting Mycobacterium tuberculosis(Mtb) infection in high school freshmen. METHODS: We concurrently administered QFT-GIT, QFT-Plus, EC skin test, and TST to first-year high school students. Blood samples were obtained for the QFT-GIT and QFT-Plus assays before the administration of the EC skin test and TST. The diagnostic values were compared. Discrepancies between the tests were quantified using Cohen's kappa coefficient. RESULTS: A total of 787 freshmen were recruited in this study. Among 787 subjects, EC was positive in 0.8%, TST in 5.3%, QFT-GIT in 1.1%, and QFT-Plus in 3.2%. Overall agreements for QFT-GIT vs. QFT-Plus, QFT-Plus TB1, and QFT-Plus TB2 were 95.7% (95% CI, 94.0-97.0), 97.3% (95% CI, 95.9-98.3), and 95.9% (95% CI, 94.3-97.2), respectively. Cohen's kappa values were 0.485 (95% CI, 0.319-0.621), 0.593 (95% CI, 0.413-0.744), and 0.451 (95% CI, 0.274-0.600). Consistency rates for QFT-GIT, QFT-Plus, EC skin test, and TST were 96.6% (95% CI, 95.0, 97.0), 92.1% (95% CI, 89.0, 94.0), 94.5% (95% CI, 92.6, 96.1), and 91.2% (95% CI, 88.8, 93.1) with Cohen's kappa values of 0.19 (95% CI, -0.01, 0.38), 0.07 (95% CI, -0.02, 0.19), 0.08 (95% CI, -0.01, 0.23), a...