Astragali Radix-Curcumae Rhizoma normalizes tumor blood vessels by HIF-1α to anti-tumor metastasis in colon cancer

作者：Yan Liang, Tingting Su, Shijiao Zhu, Ruolan Sun, Jiahui Qin, Zengyaran Yue, Xu Wang, Zhongqing Liang, Xiying Tan, Yong Bian, Fan Zhao, Decai Tang, G. George Yin · 发表于：Phytomedicine · 年份：2025 · DOI：10.1016/j.phymed.2025.156562 · 被引用次数：11 · 研究领域：Cancer, Hypoxia, and Metabolism、Angiogenesis and VEGF in Cancer、Traditional Chinese Medicine Analysis

BACKGROUND: Abnormal tumor blood vessels can significantly promote the malignant progression of tumors, prompting researchers to focus on drugs that normalize these vessels for clinical treatment. The combination of the Qi-tonifying drug Astragali Radix and the blood-activating drug Curcumae Rhizoma, referred to as AC, exhibited significant anti-tumor metastasis effects. However, the association between the anti-tumor metastasis effect of AC and its potential role in regulating tumor vascular remodeling warrants further exploration. PURPOSE: This study aimed to elucidate the mechanism through which AC induces tumor blood vessel normalization in colon cancer (CC). METHODS: The potential active components of AC were identified through UPLC-MS/MS. An orthotopic transplantation model of CC was established in BALB/c mice using the CT26-Lucifer cell line, and the effects of AC were evaluated using IVIS imaging, hematoxylin and eosin (H&E) staining, and immunohistochemistry. Network pharmacology and molecular biology analyses were employed to identify the potential direct targets of AC. Subsequently, RT-PCR and Western blotting techniques were utilized to validate the findings obtained from network pharmacology. Furthermore, ELISA and other methodologies were used to investigate glycolysis-related indicators, along with immunofluorescence technology to demonstrate changes in vascular leakage and perfusion characteristics associated with blood vessel normalization. RESULTS: We identi...