Exploration of Suitable Conditions for Shoot Proliferation and Rooting of Quercus robur L. in Plant Tissue Culture Technology

作者：Ting Wang, Hao Li, Jiu‐Jiu Zhao, Jin‐Liang Huang, Yu Zhong, Zhenfeng Xu, Fang He · 发表于：Life · 年份：2025 · DOI：10.3390/life15030348 · 被引用次数：7 · 研究领域：Plant tissue culture and regeneration、Plant Pathogens and Fungal Diseases、Plant-Microbe Interactions and Immunity

Quercus robur L., also referred to as “summer oak” or “English oak”, is an esthetically pleasing species, making it an excellent choice for street trees and gardens. Raising Quercus presents several challenges, including its long growth period, delayed germination, and inconsistent emergence. The shoot proliferation and adventitious root formation of Q. robur are crucial for establishing a tissue culture regeneration system and are vital for the successful transplantation of seedlings. To address this, experiments were conducted to assess shoot proliferation and adventitious root formation in Q. robur using various media. The shoot proliferation time, shoot proliferation coefficient, number of rooting strips, and length indicators of roots were recorded. The results indicated that a combination of 0.3 mg/L 6-Benzylaminopurine (6-BA) and 100 mg/L cefotaxime (Cef) was optimal for shoot propagation, while a solution of 0.1 mg/L 1-Naphthaleneacetic acid (NAA) and 1/2 Murashige and Skoog Medium (1/2MS) medium was most effective for root induction. This study has identified the optimal conditions for adventitious root formation and shoot proliferation in Q. robur, providing a basis for further research into propagation, germplasm conservation and genetic transformation techniques.