Multi-antigen serology and a diagnostic algorithm for the detection of arbovirus infections as novel tools for arbovirus preparedness in southeast Europe (MERMAIDS-ARBO): a prospective observational study

作者：Louella M.R. Kasbergen, Louella M R Kasbergen, Erwin de Bruin, Felicity Chandler, Louise Sigfrid, Xin Hui S Chan, Lauren Hookham, Jia Wei, Siyu Chen, Corine H. GeurtsvanKessel, Sandra Scherbeijn, Rémi N. Charrel, Nazli Ayhan, James Lee, Victor M. Corman, Chantal Reusken, Katherine Loens, Corneliu Petru Popescu, Mihaela Lupșe, Violeta Briciu, Anca Meda Văsieşiu, Pëllumb Pipero, Arjan Harxhi, Edmond Puca, Albina Ponosheci Biçaku, Maja Travar, Maja Ostojić, Rusmir Baljić, Jurica Arapović, Dragan Ledina, Đurđica Cekinović Grbeša, Ivica Čabraja, Ivan-Christian Kurolt, Stela Halichidis, Victoria Bîrluțiu, Irina Magdalena Dumitru, Ruxandra Moroti, Aleksandra Barać, Goran Stevanović, Athina Pyrpasopoulou, Vasilios Koulouras, Ljiljana Radić, Metaxia Papanikolaou, Emmanuel Roilides, Alemka Markotić, Ushma Galal, Emmanuelle Denis, Lynsey Goodwin, Lance Turtle, Simin Aysel Florescu, Hamdi Ramadani, Herman Goossens, Margareta Ieven, Christian Drosten, Peter Horby, Reina S Sikkema, Marion P G Koopmans, Louella M R Kasbergen, Louella M R Kasbergen, Erwin De Bruin, Felicity Chandler, Louise Sigfrid, Xin-Hui S Chan, Lauren Hookham, Jia Wei, Siyu Chen, Corine H GeurtsvanKessel, Sandra Scherbeijn, Remi N Charrel, Nazlı Ayhan, James L Lee, Victor M Corman, Chantal Reusken, Katherine Loens, Corneliu Petru Popescu, Mihaela Lupse, Violeta Briciu, Anca Meda Vasiesiu, Pellumb Pipero, Arjan Harxhi, Edmond Puca, Albina Ponosheci Biçaku, Maja Travar, Maja Ostojić, Rusmir Baljic, Jurica Arapović, Dragan Ledina, Đurđica Cekinović Grbeša, Ivica Čabraja, Ivan-Christian Kurolt, Stela Halichidis, Victoria Birlutiu, Irina M Dumitru, Ruxandra Moroti, Aleksandra Barac, Goran Stevanovic, Athina Pyrpasopoulou, Vasilios Koulouras, Ljiljana Betica Radić, Metaxia N Papanikolaou, Ioannis Andrianopoulos, Catalin Apostolescu, Silvia Bino, Maria Chondrogiorgi, Natalie Cleton, Daniel Codreanu, Tomasović Domagoj, Edita Dražić Maras, Mario Duvnjak, Adriana Hristea, Raluca M Hrisca, Kevin Jacobs, Ilias Iosifidis, Puškarić Ivana, Alma Gabriela Kosa, Iris Koshovari, Mandy Kuijstermans, Arsim Kurti, Santini Marija, Iosif Marincu, Julia Melchert, Pieter Moons, Visnja Mrdjen, Cristian M Niculae, Nenad Pandak, Lul Raka, Iordanis Romiopoulos, Željana Sulaver, Gratiela Tardei, Nijaz Tihic, Skuhala Tomislava, Antonija Verhaz, Mihaela Zaharia, Mačak Šafranko Željka, Ivana Vrkic, Ankica Vujovic, Emmanuel Roilides, Alemka Markotić, Ushma Galal, Emmanuelle Denis, Lynsey Goodwin, Lance Turtle, Simin Aysel Florescu, Hamdi Ramadani, Herman Goossens, Margareta Ieven, Christian Drosten, Peter W Horby, Reina S. Sikkema, Marion Koopmans · 发表于：The Lancet Infectious Diseases · 年份：2025 · DOI：10.1016/s1473-3099(24)00654-6 · 被引用次数：7 · 研究领域：Viral Infections and Vectors、Mosquito-borne diseases and control、Viral Infections and Outbreaks Research

BACKGROUND: Arboviruses are increasingly affecting Europe, partly due to the effects of climate change. This increase in range and impact emphasises the need to improve preparedness for emerging arboviral infections that often co-circulate and might have overlapping clinical syndromes. We aimed to strengthen surveillance networks for four clinically relevant arboviruses in southeast Europe. METHODS: This study reports an in-depth analysis of the MERMAIDS-ARBO prospective observational study in adults (ie, aged ≥18 years) hospitalised with an arbovirus-compatible disease syndrome in 21 hospitals in seven countries in southeast Europe over four arbovirus seasons (May 1-Oct 31, 2016-19) to obtain arbovirus prevalence outcomes. The main objectives of the MERMAIDS-ARBO study, describing the clinical management and outcomes of four arboviruses endemic to southeast Europe, including Crimean-Congo haemorrhagic fever virus (CCHFV), tick-borne encephalitis virus (TBEV), Toscana virus, and West Nile virus (WNV), are reported elsewhere. In this analysis, given the challenges associated with arbovirus diagnostics, we developed a diagnostic algorithm accounting for serology outcomes and sample timing to study arbovirus prevalence in southeast Europe. Serum samples were collected on days 0, 7, 28, and 60 after hospital admission and tested for anti-CCHFV IgG and IgM antibodies with ELISAs (confirmed with an indirect immunofluorescence test) and for IgG and IgM antibodies specific to TBEV, T...