Metagenomic analysis revealed the bioremediation mechanism of lead and cadmium contamination by modified biochar synergized with Bacillus cereus PSB-2 in phosphate mining wasteland

作者：Yuxin Zhang, Jun Peng, Ziwei Wang, Fang Zhou, Junxia Yu, Ruan Chi, Chunqiao Xiao · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1529784 · 被引用次数：19 · 研究领域：Chromium effects and bioremediation、Adsorption and biosorption for pollutant removal、Heavy metals in environment

Introduction Phosphate mining wasteland is contaminated with heavy metals, such as lead (Pb) and cadmium (Cd), which pose significant environmental risks. Ecological restoration of these lands is crucial, but limited research has focused on the remediation of heavy metal-contaminated soils using modified biochar and functional microorganisms. Methods In this study, we investigated the bioremediation of phosphate mining wasteland soil using modified biochar in combination with the phosphate-solubilizing bacterium Bacillus cereus . The effects of this synergistic approach on soil nutrient content, heavy metal immobilization, and microbial community structure were assessed. Results and discussion The results indicated that the available phosphate content in the soil increased by 59.32%. The content of extractable state Pb 2 + and Cd 2 + decreased by 65.06 and 71.26%, respectively. And the soil nutrient conditions were significantly improved. Synergistic remediation can significantly increase the diversity and abundance of soil microbial communities ( p < 0.05). Janibacter , Lysobacter , Ornithinimicrobium , Bacillus , and Salinimicrobium were the main functional flora during soil remediation, with significant correlations for the promotion of Pb 2 + and Cd 2 + immobilization and the increase of available phosphate and organic matter. ZitB , czcD , zntA , and cmtR are the major heavy metal resistance genes and regulate metabolic pathways to make microbial community functio...