Site-Specific Protein Modification via Reductive Amination of Genetically Encoded Aldehyde

作者：Lei Zhao, Zhifen Huang, Huyan Meng, Qian Liang, Xun‐Cheng Su, Weimin Xuan · 发表于：Bioconjugate Chemistry · 年份：2025 · DOI：10.1021/acs.bioconjchem.4c00538 · 被引用次数：1 · 研究领域：Chemical Synthesis and Analysis、Click Chemistry and Applications、Monoclonal and Polyclonal Antibodies Research

Aldehyde represents an extremely useful bio-orthogonal group in chemical biology and has promoted the generation of high-quality bioconjugates in therapeutics development. However, the installation of an aldehyde group on a protein and subsequent conjugation remains technically inadequate in the aspect of site choice, substrate availability, and linkage stability. Herein, we take efforts to advance the genetic incorporation of an aldehyde-containing noncanonical amino acid in E. coli and then show that reductive amination could be a useful reaction in introducing various amine-containing molecules, including peptides, into a specific site of proteins.