GraphEPN: A Deep Learning Framework for B-Cell Epitope Prediction Leveraging Graph Neural Networks

作者：Feng Wang, Xiangwei Dai, Liyan Shen, Shan Chang · 发表于：Applied Sciences · 年份：2025 · DOI：10.3390/app15042159 · 被引用次数：3 · 研究领域：vaccines and immunoinformatics approaches、Machine Learning in Bioinformatics、Glycosylation and Glycoproteins Research

B-cell epitope prediction is crucial for advancing immunology, particularly in vaccine development and antibody-based therapies. Traditional experimental techniques are hindered by high costs, time consumption, and limited scalability, making them unsuitable for large-scale applications. Computational methods provide a promising alternative, enabling high-throughput screening and accurate predictions. However, existing computational approaches often struggle to capture the complexity of protein structures and intricate residue interactions, highlighting the need for more effective models. This study presents GraphEPN, a novel B-cell epitope prediction framework combining a vector quantized variational autoencoder (VQ-VAE) with a graph transformer. The pre-trained VQ-VAE captures both discrete representations of amino acid microenvironments and continuous structural embeddings, providing a comprehensive feature set for downstream tasks. The graph transformer further processes these features to model long-range dependencies and interactions. Experimental results demonstrate that GraphEPN outperforms existing methods across multiple datasets, achieving superior prediction accuracy and robustness. This approach underscores the significant potential for applications in immunodiagnostics and vaccine development, merging advanced deep learning-based representation learning with graph-based modeling.