Dual-mode ratiometric electrochemical and turn-on fluorescent probe for reliably detecting H2O2 in Parkinson's disease serum

作者：Hui Dong, Linlin Zheng, Zuying Wang, Ke Xu, Wentian Chen, Zi Liu, Zhu Chang, Yanli Zhou, Xu Zhu, Yintang Zhang, Maotian Xu · 发表于：Sensors and Actuators Reports · 年份：2025 · DOI：10.1016/j.snr.2025.100305 · 被引用次数：17 · 研究领域：Electrochemical sensors and biosensors、Molecular Sensors and Ion Detection、Conducting polymers and applications

• A dual-mode probe Re-B for detection of H 2 O 2 were designed and synthesized. • The ratiometric electrochemical strategy based on a single probe molecule possessed a self-calibration function. • The Re-B probe exhibits a ``turn-on'' response to H 2 O 2 and maintains low background fluorescence. • The dual-mode signals are cross-validated, minimizing the risk of false positives and negatives. Accurate diagnosis and effective treatment of Parkinson's disease (PD) rely on the accurate detection of the corresponding biomarkers. The build-up of reactive oxygen species (ROS), for instance, hydrogen peroxide (H 2 O 2 ), resulting in oxidative stress inside cells is closely related to the pathogenesis of PD. In this study, two compounds, Re-B and Re-OB, were synthesized through a simple one-step reaction. Upon reacting with H 2 O 2 , the borate ester group in Re-B is selectively cleaved, restoring the phenol group. This leads to both a dual-signal ratiometric electrochemical response and a ``turn-on'' fluorescence effect. The Re-B probe demonstrates a clear ``turn-on'' electrochemical current response at −311.1 mV, followed by a ``turn-off'' response at −257.6 mV when interacting with H 2 O 2 on a single-walled carbon nanotube-modified screen-printed carbon electrode (SPCE/SWCNTs). Concurrently, the fluorescence spectrum shows a ``turn-on'' response with an emission peak at 586 nm. The fluorescence and ratiometric electrochemical methods developed in this study show exceptionally ...