SSR markers development and their application in genetic diversity of burdock (Arctium lappa L.) germplasm

作者：Yijun Su, Jiangyan Fu, Hao Xie, Zihui Huang, Yimeng Li, Yuankai Luo, Xinxing Zhou, Yangyang Li, Jian Li, Yawei Sun, Yaju Liu · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-06203-8 · 被引用次数：11 · 研究领域：Plant-derived Lignans Synthesis and Bioactivity、Sesquiterpenes and Asteraceae Studies、Genetic diversity and population structure

BACKGROUND: Arctium lappa L. is a medicinal edible homologous plant, commonly known as burdock or bardana, which belongs to the Asteraceae family and is abundant all over the world. Genetic diversity assessment is essential for A. lappa germplasm resource conservation and breeding. The assessment techniques include morphological, biochemical, and DNA marker analysis. However, the limited number of available DNA markers is insufficient to conduct related genetic diversity assessment studies. RESULTS: In this study, we conducted RNA sequencing of the A. lappa cultivar 'Yanagikawa Ideal' and developed SSR markers to characterize the genetic diversity and population structure of 56 A. lappa accessions and 8 wild relative accessions. A total of 4,851 simple sequence repeats (SSRs) loci were identified. The proportions of mono-, di- and tri-nucleotide repeat motifs were 30.40%, 21.50% and 33.10%, respectively. We developed and verified the reliability of 28 SSR core primer pairs through electronic polymerase chain reaction (ePCR) and the PCR amplification process. The polymorphism information content (PIC) values of the 28 SSR core primer pairs ranged from 0.246 to 0.848, with 14 pairs of SSR primers displaying high polymorphism (PIC > 0.5). The 28 SSR core primer pairs showed 100% mobility in Arctium tomentosum Miller and 96.43% mobility in Synurus deltoides (Aiton) Nakai, indicating their high versatility. The average Shannon information index (I) was 1.231, and the average obser...