Biomimetic Fabrication and Osteogenic Effects of E7BMP-2 Peptide Coassembly Microspheres Based α-Tricalcium Phosphate with Silk Fibroin

作者：Yan Zhu, Xin Yu, Mingxuan Hao, Yushan Wang, Gentao Fan, Hongbo Qian, Peng Jiang, Zhengdong Cai, Zhiwei He, Guangxin Zhou · 发表于：ACS Applied Bio Materials · 年份：2025 · DOI：10.1021/acsabm.4c01633 · 被引用次数：4 · 研究领域：Bone Tissue Engineering Materials、Silk-based biomaterials and applications、Protein Hydrolysis and Bioactive Peptides

The repair and reconstruction of bone defects remain a challenge in orthopedics. Inadequate mechanical qualities, poor biocompatibility, and insufficient osteoconductivity are some of the issues facing current bone healing materials. Better materials that can replicate the composition and functionality of natural bone, promote quick and full healing, and reduce the likelihood of rejection and infection are desperately needed. Bone tissue engineering, combining biomaterial scaffolds and pro-osteogenic drugs, provides support in the repair and regeneration of bone defects. The development of an effective scaffold for bone defect repair is an urgent clinical need. The present study investigates the feasibility of using microspheres based on α-tricalcium phosphate and fibroin as an osteoconductive matrix and a carrier for controlled local delivery of the E7BMP-2 peptide, in which the E7 domain confers a calcium chelation property, while the BMP-2 mimicking peptide induces bone formation. We prepared α-tricalcium phosphate/silk fibroin (α-TCP/SF) microspheres through a high voltage electric field based on the protocol of α-TCP/SF bone cement slurry. This α-TCP/SF microspheres-based system was designed for delivery vehicles of the modified BMP-2 peptide by the E7 domain to realize sustainable and steady release of the peptide. In vitro cell tests and the experimental model of cranial bone defects in rats were used to investigate the pro-osteogenic benefits. The results demonstrated...