Comparison of the capillary and venous blood plasma lipidomes: validation of self-collected blood for plasma lipidomics

作者：Ahsan Hameed, Mário G. Ferruzzi, Colin D. Kay, D. Keith Williams, Elaheh Rahbar, Andrew J. Morris · 发表于：Journal of Lipid Research · 年份：2025 · DOI：10.1016/j.jlr.2025.100755 · 被引用次数：9 · 研究领域：Metabolomics and Mass Spectrometry Studies、Mass Spectrometry Techniques and Applications、Advanced Proteomics Techniques and Applications

Venipuncture of the upper extremities is commonly used to collect blood for plasma lipidomics. However, self-administered blood collection devices such as the Tasso+™ system for capillary blood sampling and plasma separation are convenient and enable frequent sampling without a clinical blood draw. The purpose of this study is to validate Tasso+ sampling for plasma lipidomics by comparing the venous blood and Tasso+-sampled capillary blood plasma lipidomes. Lipids are proven or putative biomarkers of human health and disease and indicators of nutritional and toxicological status. Because exchange of blood components including lipids occurs in capillaries, the capillary and venous blood lipidomes might be different, which could confound use of Tasso+-sampled blood as a surrogate for venous blood plasma. Here we compared the lipidomes of Tasso+-drawn capillary blood plasma and venous blood plasma in 10 male subjects using high-resolution mass spectrometry-based lipidomics. While there was substantial interindividual variability between lipidomes, comprehensive statistical approaches with cross-validation and multiple testing adjustments showed no difference (adjusted P-value > 0.05) in lipid composition of the paired blood samples. A linear regression model with Spearman correlation analysis also showed a significant-to-near-perfect level (r = 0.95-0.99) of concordance between the samples. Aside from monoacylglycerols and cardiolipins, every class of lipid was strongly correlat...