Mass spectrometry imaging reveals spatial metabolic variation and the crucial role of uridine metabolism in liver injury caused by Schistosoma japonicum

作者：Qingkai Xue, Xiangyu Zhou, Yuyan Wang, Yiyun Liu, Xiaojing Li, Chunrong Xiong, Xinjian Liu, Yuzheng Huang · 发表于：PLoS neglected tropical diseases · 年份：2025 · DOI：10.1371/journal.pntd.0012854 · 被引用次数：4 · 研究领域：Parasites and Host Interactions、Trace Elements in Health、Liver Disease and Transplantation

Schistosomiasis is the second most important parasitic disease worldwide. Schistosomiasis japonica is a unique species endemic to southern China, and schistosomiasis is characterized by severe liver injury, inflammation, liver granuloma, and subsequent liver fibrosis. However, the pathological mechanism of this disease remains unclear. Mass spectrometry imaging (MSI) is a versatile technique that integrates the molecular specificity of mass spectrometry (MS) with spatial imaging information, which could provide an accurate method for observing disease progression. In this study, we used an air flow-assisted desorption electrospray ionization (AFADESI-MSI) platform to detect a wide range of metabolites and visualize their distribution in the liver tissue of mice infected with Schistosoma japonicum. In the negative ion mode analysis, 21 and 25 different metabolites were detected in the early and chronic stages of infection, respectively. Thirteen characteristic metabolites and 3 metabolic pathways related to disease development may be involved in the chronicity of schistosomiasis. There were more than 32 and 40 region-specific changes in the abundance of a wide range of metabolites (including carbohydrates, amino acids, nucleotides, and fatty acids) in the livers of mice at two different infection times, which also revealed the heterogeneous metabolic characteristics of the liver egg granulomas of S. japonicum. In a chronic infection model with S. japonicum, oral treatment with...