Smooth muscle contractility of laser-enucleated prostate tissues and impacts of preoperative α1-blocker treatment in patients with and without catheterization

作者：Patrick Keller, Sheng Hu, Laurenz Berger, P. Nicola, Felix Schierholz, Alexander Tamalunas, Oluwafemi Ezekiel Kale, Philipp Weinhold, Raphaela Waidelich, Christian G. Stief, Martin Hennenberg · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-88884-7 · 被引用次数：5 · 研究领域：Urinary Bladder and Prostate Research、Prostate Cancer Diagnosis and Treatment、Connexins and lens biology

Abstract Prostate smooth muscle contraction is central in treatment of voiding symptoms in benign prostatic hyperplasia (BPH). Tissues from transurethral resection of the prostate (TURP) and radical prostatectomy (RP) for prostate cancer are widely used to study contractions. However, findings are limited by traumatization in TURP, and uncertain relationship to BPH in RP tissues. This study aims to examine contractions of laser-enucleated tissues. Tissues from holmium/thulium laser enucleation (HoLEP/ThuLEP) and TURP were contracted by KCl, noradrenaline and electric field stimulation (EFS) in an organ bath. Contractions were compared to RP tissues in previous studies. KCl-induced contractions averaged 2.5 mN, 0.7 mN and 3.3 mN in tissues from HoLEP/ThuLEP, TURP and RP, with non-responsive tissues included (2.4% HoLEP/ThuLEP, 37% TURP). Maximum EFS-induced contractions (E max ) averaged 47% of KCl in HoLEP/ThuLEP tissues, 27% in TURP tissues, and 68–235% in 21 previous studies with RP tissues. E max values for noradrenaline averaged 99.7% in HoLEP/ThuLEP tissues, 56% in TURP tissues, and ranged from 92 to 260% in RP tissues. Preoperative α 1 -blocker treatment reduced EFS- and noradrenaline-induced contractions, and increased EC 50 values for noradrenaline in laser-enucleated, catheterized patients, but not in patients without catheterization. Also, the ex vivo application of α 1 -blockers increased the EC 50 values for noradrenaline and reduced E max for EFS. Laser-enucleate...