Qingyi decoction and its active ingredients ameliorate acute pancreatitis by regulating acinar cells and macrophages via NF-κB/NLRP3/Caspase-1 pathways

作者：Yu An, Zhengwei Tu, Ao Wang, Wenfeng Gou, Huijuan Yu, Xuefei Wang, Feifei Xu, Yanli Li, Cong Wang, Jinan Li, Mengyue Zhang, Mengbo Xiao, Ying Di, Wenbin Hou, Yunfeng Cui · 发表于：Phytomedicine · 年份：2025 · DOI：10.1016/j.phymed.2025.156424 · 被引用次数：26 · 研究领域：Pancreatitis Pathology and Treatment、Inflammasome and immune disorders、Phagocytosis and Immune Regulation

BACKGROUND AND PURPOSE: Macrophage infiltration and activation is a critical step during acute pancreatitis (AP). NLRP3 inflammasomes in macrophages plays a critical role in mediating pancreatic inflammatory responses. Qing-Yi Decoction(QYD)has been used for many years in clinical practice of Nankai Hospital combined with traditional Chinese and western medicine treatment of acute pancreatitis. Although QYD has a well-established clinical efficacy, little is known about its bioactive ingredients, how they interact with different therapeutic targets and the pathways to produce anti-inflammatory effects. Here, we elucidate the therapeutic effects of QYD against acute pancreatitis and reveal its mechanism of action. METHODS: The main components of QYD were identified using UHPLC-Q-Orbitrap MS. Network pharmacology was employed to predict potential therapeutic targets and their mechanisms of action. C57BL/6 mice were randomly divided into control group, model group, low, medium and high dose (6, 12, 24 g/kg) QYD groups, with 10 mice in each group. The therapeutic effect of QYD on cerulein-induced acute pancreatitis. (CER-AP) was evaluated by histopathological score, immunohistochemistry, serum amylase and cytokines detection by ELISA. The protein expressions of MyD88/NF-κB/NLRP3 signaling pathway were detected by Western blotting. Along with molecular docking of key bioactive compounds and targets, RAW264.7 cells stimulated with 1μg/ml LPS is used to screen components with more p...