LncRNA ‐ MEG3 Mediated Diabetic Cerebral Ischemia–Reperfusion Injury‐Induced Apoptosis via Modulating Interaction Between Annexin A2 and Akt in Mitochondria

作者：Wanqing Zhou, Chongyi Tan, Di Xiong, Cheng Chen, Yanfei Zhao, Yongqiu Xie, Bei Sun, Zhihua Wang, Pingping Xia, Zhi Ye · 发表于：CNS Neuroscience & Therapeutics · 年份：2025 · DOI：10.1111/cns.70242 · 被引用次数：7 · 研究领域：S100 Proteins and Annexins、Cancer-related molecular mechanisms research、Cardiac Ischemia and Reperfusion

BACKGROUND: In clinical domains, encompassing neurosurgery and macrovascular cardiac procedures, certain interventions result in cerebral ischemia- reperfusion injury (CIRI). Diabetes mellitus (DM) increases the risk of CIRI and worsens the severity of neurological impairment. It was documented that lncRNA-MEG3 contributed to the pathogenesis of CIRI. However, the pivotal significance of lncRNA-MEG3 in diabetic CIRI has never been studied. AIMS: This study's aims were two-fold, to (1) figure out the influence of lncRNA-MEG3 on neurological dysfunction subsequent to diabetic cerebral ischemic injury, (2) elucidate its potential role in mitochondria-related apoptosis via modulating the Anxa2 signaling pathway. MATERIALS AND METHODS: We mainly collected plasma from clinical patients to measure the expression of lncRNA-MEG3, and explored the molecular mechanism of lncRNA-MEG3 in CIRI combined with DM by immunofluorescence, western blot, co-ip and other molecular biology experiments in rat MACO+DM model and cellular OGD/R+HG model. RESULTS: LncRNA-MEG3 expression in DM+AIS cases was remarkably higher than that in cases with AIS and healthy controls. Moreover, lncRNA-MEG3 expression was strongly linked to the National Institutes of Health Stroke Scale (NIHSS) score. Additionally, the findings unveiled that lncRNA-MEG3 depletion alleviated neurological impairments following CIRI in diabetic rats, and cellular death resulted from Oxygen-glucose deprivation (OGD) plus hyperglycemic re...