Synthesis and Bioactivity of Selenium Nanoparticles From Tussilago farfara L. Polysaccharides: Antioxidant Properties and MCF‐7 Cell Inhibition

作者：Ke Guo, Xin‐Xin Yang, JinRong Wang, Wei Chang, Simei Liu, Siqi Zhang, Tingting Zhang, Hao Yan, Yafeng Yan, Jie Wang, Xiping Chen, Canqing Yu, Guoquan Wang, Peng Zhao · 发表于：Chemistry & Biodiversity · 年份：2025 · DOI：10.1002/cbdv.202402677 · 被引用次数：6 · 研究领域：Selenium in Biological Systems、Plant Toxicity and Pharmacological Properties、Moringa oleifera research and applications

The present study reports the synthesis of selenium nanocomplexes (Se-TFPs) using purified polysaccharides from Tussilago farfara L. (coltsfoot). It evaluates its structural characteristics, physicochemical properties, and inhibitory effects of Michigan Cancer Foundation-7 (MCF-7) breast cancer cells. The influence of processing conditions on nanoparticle size and stability at 25°C was assessed using particle size and zeta potential measurements. The Se-TFPs were synthesized by optimizing the processing conditions via response surface methodology, yielding nanoparticles with a selenium (Se)-to-polysaccharide mass ratio of 1:13.5, a Se-to-ascorbic acid molar ratio of 1:4.5, a selenite concentration of 10.7 mM, and a reaction time of 4.4 h. The resulting Se-TFPs had an average particle size of 107.2 nm and a zeta potential of -35.1 mV. Structural and physicochemical analyses confirmed successful nanoparticle formation. Compared to TFPs, Se-TFPs exhibited significantly enhanced scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl radicals, and superoxide anion radicals. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, flow cytometry, and cell cycle apoptosis analysis revealed that Se-TFPs effectively inhibited MCF-7 cell proliferation at the S phase, with an IC50 value of 119.62 µg/mL.