Lactobacillus rhamnosus GG ameliorates atherosclerosis via suppression of oxidative stress and inflammation by reshaping the gut microbiota

作者：Yajuan Liu, Zhixia Bai, Ru Yan, Junbai Ma, Liting Wang, Yiwei Li, Yuanyuan Liu, Huiyan Ma, Ting Wang, Libo Yang, Jian Liu, Wenke Shen, Xiaoxia Zhang, Shaobin Jia, Hao Wang · 发表于：Biochemical and Biophysical Research Communications · 年份：2025 · DOI：10.1016/j.bbrc.2025.151417 · 被引用次数：9 · 研究领域：Gut microbiota and health、Probiotics and Fermented Foods、Atherosclerosis and Cardiovascular Diseases

OBJECTIVE: With growing awareness of probiotics' benefits, more studies are exploring their efficacy and mechanisms in reducing atherosclerosis (AS). This study aimed to investigate the potential therapeutic effects of Lactobacillus rhamnosus GG (LGG) on atherosclerotic mice and underlying mechanisms. DESIGN: and C57BL/6J mice received normal saline as controls. After 15 weeks, en face Oil Red O staining and aortic sinus morphometry were used to assess the effects of LGG intervention on AS. The expression of the Nrf2/HO-1 pathway, along with oxidative stress and inflammation, was measured in the aortic sinus, aortas, or plasma. Immune cells were analyzed by flow cytometry. 16S rRNA gene sequencing analysis evaluated structural changes in the intestinal microbiota. RESULTS: mice exhibited disrupted intestinal flora. Nonetheless, LGG intervention restored their intestinal flora to a composition resembling that of C57BL/6J mice, thereby increasing the abundance of beneficial bacteria. CONCLUSION: LGG significantly attenuates AS by reducing oxidative stress and inflammation probably via activating the Nrf2/HO-1 pathway. Remarkably, LGG modulates gut microbiota, further enhancing its protective efficacy against AS.