Transcriptome signature in the blood of neuromyelitis optica spectrum disorder under steroid tapering

作者：R. Yamamura, Makoto Kinoshita, Yoshiaki Yasumizu, Tomohiro Yata, Keigo Kihara, Daisuke Motooka, Naoyuki Shiraishi, Yasuko Sugiyama, Shohei Beppu, Hisashi Murata, Naoshi Koizumi, Itsuki Sano, Toru Koda, Tatsusada Okuno, Hideki Mochizuki · 发表于：Frontiers in Immunology · 年份：2025 · DOI：10.3389/fimmu.2025.1508977 · 被引用次数：3 · 研究领域：Multiple Sclerosis Research Studies、Neuroinflammation and Neurodegeneration Mechanisms、Spondyloarthritis Studies and Treatments

Background: The advent of biologics has significantly transformed treatment strategies for neuromyelitis optica spectrum disorder (NMOSD). However, there are no biomarkers that predict relapses associated with steroid tapering; therefore, it is critical to identify potential indicators of disease activity. In this study, we collected peripheral blood mononuclear cells (PBMCs) from NMOSD patients during steroid tapering and performed bulk RNA sequencing to analyze changes in immune dynamics caused by steroid reduction. Methods: PBMCs were collected at 3-5 timepoints from 10 NMOSD patients at our hospital (including one relapse case), and bulk RNA sequencing was performed. All patients were positive for anti-AQP4 antibodies and had no history of biologic use. Results: In one relapsed patient, gene groups with decreased expression at relapse were observed predominantly in monocytes, with upregulation in anti-inflammatory pathways such as IL-10, while the upregulated genes were related to interferon signaling. Moreover, after steroid tapering, in non-relapsed patients, genes with increased expression were enriched in inflammatory pathways, represented by interferon signaling, while genes with decreased expression were enriched in pathways related to IL-10 and glucocorticoid receptors. Weighted gene co-expression network analysis identified modules that correlated with steroid dosage, and the modules inversely correlated with steroid dosage were enriched in monocytes, with marked ...