Genome-wide association analysis for pollen viability under heat stress in peanut

作者：Hrishikesh P. Ingole, Gautam Saripalli, Zachary T. Jones, Manikannan Parthiban, Johnson Toyinbo, Sruthi Narayanan, Reyazul Rouf Mir, Sachin Rustgi · 发表于：Plant Stress · 年份：2025 · DOI：10.1016/j.stress.2025.100760 · 被引用次数：5 · 研究领域：Peanut Plant Research Studies、Agricultural pest management studies、Plant pathogens and resistance mechanisms

• Pollen viability under heat stress is genetically controlled. • A major locus for pollen viability under heat stress was identified through GWAS. • This QTL coincided with a previously identified QTL hotspot for heat stress traits. • This QTL has implications for breeding heat-tolerant crops. Peanut is one of the most important crops, providing nutrition and food security to millions worldwide. Pollen viability (PV) is a major determinant of yield in crops and is adversely impacted by heat stress. This study aimed to identify molecular markers associated with sustained PV under heat stress. We evaluated 72 genetically non-redundant genotypes from the U.S. peanut mini-core collection for PV under heat stress and compared them to the PV of "Georgia Green," a runner-type peanut variety used as a control. Seventy-two genotypes were grown under optimal conditions (28/22 °C day/night temperatures with a 16 h photoperiod). Once the plants reached the five-leaf stage, heat stress was applied for two weeks by raising the daytime temperature to 38 °C and the nighttime temperature to 28 °C. Un-opened flowers were collected and assayed for PV through in vitro pollen germination. PI 200441 from Japan exhibited the highest PV, while PI 504614 from Colombia showed the lowest. A genome-wide association study for PPV (percent pollen viability) under heat stress identified a marker on chromosome 20. Haplotype analysis revealed a 6 kb region, designated qPPVA20 , containing three candidate ge...