Research on gene editing and immunosuppressants in kidney xenotransplantation

作者：Jia-Jiao Luo, Congwen Bian, Min Liu, Yuan Fang, Jin Li, Rui Yu, Hanfei Huang · 发表于：Transplant Immunology · 年份：2025 · DOI：10.1016/j.trim.2025.102184 · 被引用次数：4 · 研究领域：Xenotransplantation and immune response、Virus-based gene therapy research、CRISPR and Genetic Engineering

Gene-edited pig organ transplantation can solve the serious shortage of human donor organs. Currently, xenotransplantation is rapidly developing and has made significant breakthroughs. The use of GTKO (Gal knockout) pigs is an important step forward. The subsequent knockout of three genes combined with the transfer of immune-related genes effectively prolonged the survival time of non-human primate (NHP) transplantation in xenotransplantation. Due to the success of allogeneic kidney transplantation on NHP, this gene editing protocol was recently applied to clinical patients. Two patients underwent allogeneic kidney transplantation and survived for 51 days and 47 days. Exceeding the hyperacute rejection period proves that appropriate gene editing strategies and the combination of immunosuppressive agents contribute to the success of xenotransplantation. To further enhance the feasibility of pig kidney xenograft, this article mainly explores the effects of the NHP xenograft gene editing scheme and immunosuppressants on prolonging transplant survival time. • We have summarized the gene editing strategies and combination of immunosuppressive agents for xenotransplantation in recent years, as well as their survival time. • We discussed the role of each gene and the combination of related genes in prolonging the survival time of transplants. • In addition, we also discussed the problems faced by xenotransplantation in recent times and the direction of its future development.