Solution-Gated Thin Film Transistor Biosensor-Based SnO 2 Amorphous Film for Label-Free Detection of Epithelial Cell Adhesion Molecules

作者：Yaxing Zhang, Zhiwei Cai, Rong Zou, Ruling Wang, Runan Tan, Lei Wang, Yuxiang Wu, Hanping He, Yunbin He, Gang Chang · 发表于：ACS Sensors · 年份：2025 · DOI：10.1021/acssensors.4c03073 · 被引用次数：10 · 研究领域：Analytical Chemistry and Sensors、Nanowire Synthesis and Applications、Thin-Film Transistor Technologies

Epithelial cell adhesion molecule (EpCAM) was considered to be an important marker of multiple tumors, and its high expression is closely related to the early diagnosis and treatment of tumors. At present, metal oxide semiconductors have become a key component of biosensor and bioelectronics technology. Tin oxide shows great potential for development because of its nontoxic, nonpolluting, low price, and excellent electrical properties. In this study, a novel SnO 2 solution-gated thin film transistor (SGTFT) biosensor for the specific detection of EpCAM was successfully developed using SnO 2 film prepared by the sol–gel method as the channel material. By selecting the optimal thickness of 100 nm SnO 2 film as the channel material, the transconductance value ( g m ) reached 1432 μS, and the threshold voltage ( V th ) remained stable at 0.288 V. In order to achieve qualitative and quantitative detection of EpCAM, SnO 2 films were subjected to a specific chemical treatment to fix the aptamer. Through a specific recognition between the aptamer and EpCAM, the gate voltage changes were triggered to regulate the channel current of the device. FE-SEM, EIS, XPS, and electrical performance tests were employed to track and measure the modification process. Based on the optimizations described above, the prepared SGTFT exhibited high detection sensitivity (14.6 mV·dec –1 ), the limit of detection (LOD) down to 24.4 pg/mL, and the calibration curves in the range of 0.02 ng/mL–500 ng/mL for...