Risk assessment of antibiotic resistance genes in rhizosphere soil during tomato growth under bio-control bacterial inoculation

作者：Jianjie Li, Yuchan Li, Na Zhang, Zongzhuan Shen, Biao Shen, Li Rong, Xuhui Deng, Qirong Shen, Joana Falcão Salles · 发表于：Journal of Cleaner Production · 年份：2025 · DOI：10.1016/j.jclepro.2025.144911 · 被引用次数：7 · 研究领域：Pharmaceutical and Antibiotic Environmental Impacts、Plant-Microbe Interactions and Immunity、Composting and Vermicomposting Techniques

The rhizosphere is critical for interactions within the plant microbiome and serves as a key environment for the growth of bacteria that produce or resist antimicrobials. This environment facilitates the strengthening of antibiotic resistance genes (ARGs). Although applying antimicrobial-producing microorganisms helps to reduce the incidence of soil-borne diseases, little is known about their impact on ARG dynamics throughout the plant growth period. Rhizosphere soils from organic fertilizer (OF) and organic fertilizer + Bacillus amyloliquefaciens T-5 (OF + T5) from a long-term field trial were chosen to assess the ARG risk under the input of biocontrol agents during all stages of plant growth. Metagenomic sequencing was used to quantify the dynamics of ARGs, mobile genetic elements (MGEs), and microorganisms harboring these genetic elements. A microcosm pot experiment was then conducted in which tomato plants were cultivated with different concentrations of B. amyloliquefaciens (10 3 , 10 5 , 10 7 , and 10 9 cells mL −1 ) to verify the results. The metagenomic data indicated that during the early stage of plant growth, the inoculated biocontrol agent, along with other stimulated beneficial microbes, such as Pseudomonas spp. and Streptomyces spp., which contain ARGs, increased the risk of ARG enrichment in the rhizosphere. However, as plant growth progressed and pathogenic bacteria surged, these beneficial microbes suppressed the increase in the abundance of Ralstonia solanac...