Targeted regulation of sterol biosynthesis genes according to perturbations in ergosterol biosynthesis in fungi

作者：Ping Yu, Mi Zhou, Deshui Yu, Z D Zhang, Shuting Ye, Yifa Yu, Xianyun Sun, Shaojie Li, Chengcheng Hu · 发表于：Journal of Advanced Research · 年份：2025 · DOI：10.1016/j.jare.2025.01.046 · 被引用次数：16 · 研究领域：Fungal and yeast genetics research、Fungal Biology and Applications、Mycorrhizal Fungi and Plant Interactions

A proposed model for the targeted regulation of sterol biosynthesis in Neurospora crassa . Perturbations in different steps of the ergosterol biosynthesis pathway lead to the accumulation of specific sterol intermediates and activate distinct transcription factors to regulate the corresponding ergosterol biosynthesis genes, thereby maintaining sterol homeostasis. The accumulation of specific sterol intermediates is proposed to trigger the activation of the responses. • A strategy distinct from ergosterol-mediated feedback regulation for sterol biosynthesis in fungi was introduced. • Sterol biosynthesis genes are separately activated by distinct transcription factors according to biosynthesis disruptions. • Transcription factors required for ergosterol-mediated feedback regulation are also essential for the targeted activation. • Transcription factors regulating sterol biosynthesis undergo functional rewiring to meet specific regulatory demands. • The targeted regulation of sterol biosynthesis may enhance fungal viability within complex environmental contexts. The synthesis and regulation of ergosterol are vital for fungal growth and stress adaptation. While ergosterol-mediated feedback regulation is a recognized mechanism controlling sterol biosynthesis in fungi, prior research suggests the presence of additional regulatory mechanisms. However, the specifics of the alternative regulatory mechanisms have not been systematically investigated. We proposed that a regulatory netwo...