Exogenous Coreactant-Free Electrocatalytic Reactive Oxygen Species-Driven Dual-Signal Molecularly Imprinted Electrochemiluminescence Sensor for the Detection of Trenbolone

作者：Yongqiang Li, Kaida Kuang, Yang Chen, Chen Xuan, Qinghua Cheng, Ya Li, Lijuan Liang, Nengqin Jia · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.4c06850 · 被引用次数：10 · 研究领域：Advanced biosensing and bioanalysis techniques、Electrochemical sensors and biosensors、Electrochemical Analysis and Applications

Conventional dual-signal electrochemiluminescence (ECL) sensors feature high sensitivity and reliability, but the involvement of coreactants inevitably results in a complex configuration and shows reproducibility risk. Here, we propose an exogenous coreactant-free dual-signal platform, comprising luminol (anodic luminophore), CdSe quantum dots (cathodic luminophore), and Co 3 O 4 /Ti 3 C 2 electrocatalyst (coreaction promoter). At different redox potentials, Co 3 O 4 /Ti 3 C 2 induces water oxidation and oxygen reduction to produce • OH and O 2 •– radicals, which subsequently drive cathodic and anodic ECL emission, respectively. The dual-signal ECL pathways are confirmed by reactive oxygen species (ROS) scavenging, and the effect of dissolved oxygen is excluded. By integrating with molecular imprinting technology, this dual-signal ECL sensor is employed for the quantitative detection of trenbolone. It exhibits high sensitivity, broad linear range (1.0 × 10 1 to 1.0 × 10 8 fg mL –1 ), low detection limit, and detection capability of real samples. This work presents a new endogenous ROS-driven dual-signal ECL sensor that excludes the use of exogenous coreactants and offers new insights into ROS generation in a coreactant-free sensor design.