Integrating single-cell RNA and T cell/B cell receptor sequencing with mass cytometry reveals dynamic trajectories of human peripheral immune cells from birth to old age

作者：Yufei Wang, Ronghong Li, Renyang Tong, Taiwei Chen, Mingze Sun, Lingjie Luo, Zheng Li, Yifan Chen, Yichao Zhao, Chensheng Zhang, Wei Lin, Wei Lin, Haoyan Chen, Kun Qian, Alex F. Chen, Junling Liu, Lei Chen, Bin Li, Feng Wang, Li Wang, Bing Su, Jun Pu · 发表于：Nature Immunology · 年份：2025 · DOI：10.1038/s41590-024-02059-6 · 被引用次数：113 · 研究领域：Single-cell and spatial transcriptomics、T-cell and B-cell Immunology、Atherosclerosis and Cardiovascular Diseases

MAIT cells, which reached their peaks of relative abundance, clonal diversity and antibacterial capability in adolescents and then gradually tapered off. Interestingly, we identified and experimentally verified a previously unrecognized 'cytotoxic' B cell subset that was enriched in children. Finally, an immune age prediction model was developed based on lifecycle-wide single-cell data that can evaluate the immune status of healthy individuals and identify those with disturbed immune functions. Our work provides both valuable insights and resources for further understanding the aging of the immune system across the whole human lifespan.