Generation of novel bpm6 and dmr6 mutants with broad‐spectrum resistance using a modified CRISPR/Cas9 system in Brassica oleracea

作者：Yulun Zhang, Yulun Zhang, Jinhui Liu, Yingjie Li, Hongxue Ma, Jialei Ji, Yong Wang, Mu Zhuang, Limei Yang, Zhiyuan Fang, Jun Li, Chao Zhang, Liwang Liu, Marina Lebedeva, Vasiliy Taranov, Yangyong Zhang, Yangyong Zhang, Honghao Lv · 发表于：Journal of Integrative Plant Biology · 年份：2025 · DOI：10.1111/jipb.13842 · 被引用次数：12 · 研究领域：CRISPR and Genetic Engineering、Insect Resistance and Genetics、Plant Virus Research Studies

Using an optimized CRISPR/Cas9 system to knock out the BTB-POZ and MATH domain gene BoBPM6 and the DOWNY MILDEW RESISTANCE 6 gene in Brassica oleracea resulted in new lines with broad-spectrum disease resistance.