Multi-omics interpretation of how lysophospholipids regulate hepatic lipid metabolism and immunity in hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus Lanceolatu) fed high-lipid diets

作者：Hongjin Deng, Jiacheng Zhang, Weixing Liang, Beiping Tan, Shuyan Chi · 发表于：Animal nutrition · 年份：2025 · DOI：10.1016/j.aninu.2024.11.021 · 被引用次数：5 · 研究领域：Aquaculture Nutrition and Growth、Aquaculture disease management and microbiota、Physiological and biochemical adaptations

This experiment explored the effects of lysophospholipids on hybrid grouper (initial average weight: 22.65 ± 0.03 g) hepatic lipid metabolism and immunity at the transcriptome and metabolome levels. A total of 375 hybrid groupers were divided into 5 groups of 3 replicates of 25 fish each. Five isoproteic and isolipidic diets were formed by adding 0 (L 0 , control group), 0.05% (L 0.05 ), 0.1% (L 0.1 ), 0.15% (L 0.15 ) and 0.2% (L 0.2 ) lysophospholipids, respectively. After 8 wk, compared to the control group, weight gain rate, specific growth rate and protein deposition rate were significantly higher ( P < 0.05), and the feed conversion ratio was significantly lower in the L 0.1 group ( P = 0.011). The total cholesterol and triglyceride contents in the serum and liver of the fish in the L 0.1 group were significantly lower than those in the L 0 group ( P < 0.05). Compared to the L 0 group, catalase and superoxide dismutase activities were substantially higher, and malondialdehyde content was considerably lower in the L 0.1 group ( P < 0.05); complement 3, lysozyme, acid phosphatase, and alkaline phosphatase levels in the L 0.1 group were significantly higher than those in the L 0 group ( P < 0.05). The hepatic lipoprotein lipase activity and gene expression in the L 0.1 group were significantly higher whereas fatty acid synthase activity and gene expression were significantly lower than those in the L 0 group ( P < 0.05). The gene expression and activities of hepatic catalas...