Establishment of a simple Agrobacterium rhizogenes-mediated hairy root transformation system in sour jujube

作者：Xiaoya Yang, Asia Maqbool, Jiaqi Zang, Yahong Niu, Zhiguo Liu, Lixin Wang, Mengjun Liu · 发表于：Scientia Horticulturae · 年份：2025 · DOI：10.1016/j.scienta.2025.113984 · 被引用次数：6 · 研究领域：Plant tissue culture and regeneration、Plant Molecular Biology Research、Plant Reproductive Biology

• The hypocotyls of 2-week-old sour jujube seedlings infested with 0 mM AS, which were cultivated at dark condition for 12 h, the transformation efficiency was the highest. • GUS histochemical staining and qRT-PCR analysis showed that the transgenic ZjMAPKK4 hairy roots were successfully obtained. • The transformation efficiency reached 0.66 %. Jujube ( Ziziphus jujuba Mill.) is an important fruit tree in China. However, due to the high heterozygosity of jujube, the gene transformation system is not maturely established. With the development of A. rhizogenes mediated transformation technology, it was successfully applied to genetic transformation in different woody plant species, which can be employed for gene transformation in jujube. Thus, in order to establish an efficient transformation system for jujube, we obtained hairy roots by infecting sour jujube seedlings with A. rhizogenes which contained 35S::GUS-ZjMAPKK4 vector under a non-sterile environment, and different treatments on the induction of 35S::GUS-ZjMAPKK4 hairy roots. The results showed that the hypocotyls of 2-week-old sour jujube seedlings infested with 0 mM AS concentration, which were cultivated at dark condition for 12 h, the transformation efficiency was the highest that the transformed gene hairy roots were successfully induced. Furthermore, to confirm the whether ZjMAPKK4 integrated into genome of the hairy roots, the GUS histochemical staining and qRT-PCR analysis were conducted. It showed that the tra...